EHMT1 Polyclonal antibody

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  IP experiment of K-562 using 55436-1-AP

  IP result of anti-EHMT1 (IP:55436-1-AP, 4ug; Detection:55436-1-AP 1:500) with K-562 cells lysate 1360 ug.

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  IF Staining of HeLa using 55436-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EHMT1 antibody (55436-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

EHMT1 Polyclonal Antibody for IF/ICC, IP, ELISA

Cat No. 55436-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, ELISA

Synonyms

DEL9q34, EHMT1, Eu HMTase1, EUHMTASE1, FP13812, G9a like protein 1, GLP, GLP1, H3 K9 HMTase 5, KIAA1876, KMT1D, Lysine N methyltransferase 1D

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Tested Applications

• Positive IP detected in: K-562 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55436-1-AP targets EHMT1 in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: euchromatic histone-lysine N-methyltransferase 1
• Calculated Molecular Weight: 141 kDa
• Observed Molecular Weight: 150-170 kDa
• GenBank Accession Number: NM_024757
• Gene Symbol: EHMT1
• Gene ID (NCBI): 79813
• RRID: AB_3670252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H9B1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EHMT1, also known as GLP (G9a-like protein), encodes a histone lysine methyltransferase that controls brown adipose cell fate, adaptive thermogenesis, and glucose homeostasis in vivo (PMID: 24196706). EHMTs, along with LSD1 (an H3K4 demethylase), are components of the REST complex, which silences neuronal pathway genes in non-neuronal cells. EHMT1 is mainly responsible for the monomethylation and dimethylation of histone H3 lysine 9 (H3K9), forming a heteromeric complex with EHMT2 (G9a) in euchromatin (PMID: 34214254, 37663929).