EGLN3 Recombinant antibody

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  IF Staining of HepG2 using 83321-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EGLN3 antibody (83321-1-RR, Clone: 240165A6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of MCF-7 using 83321-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Egln3 Recombinant Antibody (83321-1-RR, Clone:240165A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A549 using 83321-1-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Egln3 Recombinant Antibody (83321-1-RR, Clone:240165A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83321-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83321-1-RR against Human EGLN3 were performed. The affinity constant is 1.82 nM.

EGLN3 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 83321-1-RR

Clone No.240165A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

PHD3, HIF prolyl hydroxylase 3, HIF PH3, Egl nine homolog 3, EC:1.14.11.29

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: A549 cells, MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83321-1-RR targets EGLN3 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EGLN3 fusion protein Ag27464
• Full Name: egl nine homolog 3 (C. elegans)
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC010992
• Gene Symbol: EGLN3
• Gene ID (NCBI): 112399
• RRID: AB_3670987
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H6Z9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.