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  • KD/KO Validated

EGLN3/PHD3 Polyclonal antibody

EGLN3/PHD3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 18325-1-AP


Egl nine homolog 3, EGLN3, HIF PH3, HIF prolyl hydroxylase 3, HIFPH3, HPH 1, HPH 3, PHD3

Tested Applications

Positive WB detected inA375 cells, HEK-293T cells, HEK-293 cells, HT-1080 cells, human placenta tissue
Positive IP detected inA375 cells
Positive IHC detected inhuman brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18325-1-AP targets EGLN3/PHD3 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EGLN3/PHD3 fusion protein Ag13197
Full Name egl nine homolog 3 (C. elegans)
Calculated Molecular Weight 27 kDa
Observed Molecular Weight27-30 kDa
GenBank Accession NumberBC010992
Gene Symbol EGLN3
Gene ID (NCBI) 112399
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.


Product Specific Protocols
WB protocol for EGLN3/PHD3 antibody 18325-1-APDownload protocol
IHC protocol for EGLN3/PHD3 antibody 18325-1-APDownload protocol
IP protocol for EGLN3/PHD3 antibody 18325-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao

Cancer Res

Prolyl Hydroxylase 3 Attenuates MCL-1-Mediated ATP Production to Suppress the Metastatic Potential of Colorectal Cancer Cells.

Authors - Praveenkumar Radhakrishnan

Mol Ther

WDR5 facilitates EMT and metastasis of CCA by increasing HIF-1α accumulation in Myc-dependent and independent pathways.

Authors - Tianli Chen
  • KD Validated

Asian J Androl

CHD1 deletion stabilizes HIF1α to promote angiogenesis and glycolysis in prostate cancer

Authors - Yu-Zhao Wang


A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma.

Authors - Zhirui Zeng

Med Oncol

Overexpression of the HIF hydroxylase PHD3 is a favorable prognosticator for gastric cancer.

Authors - Su Changlei C