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PHD2/EGLN1 Monoclonal antibody
PHD2/EGLN1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse , Rat , Pig and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1A2F1
Cat no : 66589-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, SH-SY5Y cells, pig brain tissue, HEK-293 cells |
Positive IHC detected in | human testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
Product Information
66589-1-Ig targets PHD2/EGLN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse , Rat , Pig samples.
Tested Reactivity | Human, Mouse , Rat , Pig |
Cited Reactivity | mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | egl nine homolog 1 (C. elegans) |
Calculated Molecular Weight | 46 kDa |
Observed Molecular Weight | 46 kDa, 44 kDa, 36 kDa |
GenBank Accession Number | NM_022051 |
Gene Symbol | PHD2/EGLN1 |
Gene ID (NCBI) | 54583 |
RRID | AB_2881949 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.
Protocols
Product Specific Protocols | |
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WB protocol for PHD2/EGLN1 antibody 66589-1-Ig | Download protocol |
IHC protocol for PHD2/EGLN1 antibody 66589-1-Ig | Download protocol |
IF protocol for PHD2/EGLN1 antibody 66589-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Cell Dev Biol Hypoxia-Inducible Factor 2-Alpha Mediated Gene Sets Differentiate Pulmonary Arterial Hypertension. | ||
J Transl Med Inhibition of OGFOD1 by FG4592 confers neuroprotection by activating unfolded protein response and autophagy after ischemic stroke
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