PHD2/EGLN1 Polyclonal antibody

PHD2/EGLN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20368-1-AP

Synonyms

EGLN1, PHD2, HIF-PH2, EGLN 1, ECYT3

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Validation Data Gallery

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  WB analysis of HEK-293 using 20368-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20368-1-AP (PHD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 20368-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20368-1-AP (PHD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20368-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20368-1-AP (PHD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20368-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20368-1-AP (PHD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20368-1-AP (PHD2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20368-1-AP (PHD2 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20368-1-AP (PHD2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20368-1-AP (PHD2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20368-1-AP (PHD2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20368-1-AP (PHD2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20368-1-AP (PHD2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 20368-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20368-1-AP (PHD2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 20368-1-AP

  Immunofluorescent analysis of HepG2 cells, using EGLN1 antibody 20368-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse pancreas tissue, HepG2 cells
• Positive IHC detected in: human pancreas tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

20368-1-AP targets PHD2/EGLN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: egl nine homolog 1 (C. elegans)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa, 36 kDa
• GenBank Accession Number: NM_022051
• Gene Symbol: PHD2/EGLN1
• Gene ID (NCBI): 54583
• RRID: AB_10695181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.

Publications

Species	Application	Title
human	WB	Oncogene; iASPP is essential for HIF-1α stabilization to promote angiogenesis and glycolysis via attenuating VHL-mediated protein degradation.; Authors - Dong Zhao
human	WB	Asian J Androl; CHD1 deletion stabilizes HIF1α to promote angiogenesis and glycolysis in prostate cancer; Authors - Yu-Zhao Wang