PHD2/EGLN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PHD2/EGLN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 19886-1-AP

Synonyms

EGLN1, PHD2, HIF-PH2, EGLN 1, ECYT3

---

Validation Data Gallery

• 

  WB analysis using 19886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 19886-1-AP

  IP result of anti-PHD2/EGLN1 (IP:19886-1-AP, 4ug; Detection:19886-1-AP 1:5000) with HEK-293 cells lysate 1280 ug.

• 

  IHC staining of human liver cancer using 19886-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19886-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19886-1-AP (PHD2/EGLN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 19886-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PHD2/EGLN1 antibody (19886-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

---

Tested Applications

• Positive WB detected in: HCT 116 cells, mouse kidney tissue, rat heart tissue, HEK-293 cells, HEK-293T cells, HepG2 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 2 publications below

Product Information

19886-1-AP targets PHD2/EGLN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHD2/EGLN1 fusion protein Ag13706
• Full Name: egl nine homolog 1 (C. elegans)
• Calculated Molecular Weight: 426 aa, 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC005369
• Gene Symbol: PHD2/EGLN1
• Gene ID (NCBI): 54583
• RRID: AB_10641986
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGLN1(Egl nine homolog 1) is also named as HIF-PH2,HPH-2,PHD2.It is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. EGLN1 plays a critical role in regulating HIF levels in EPO-producing cells in humans(PMID:16407130).Defects in EGLN1 are the cause of familial erythrocytosis type 3 (ECYT3).This antibody is specific to EGLN1.

Publications

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao ; KO Validated
	WB,IHC	Environ Int; HIF-1α/m6A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity; Authors - Xiaowei Cong
	WB	Cell Mol Life Sci; Iron increases lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the HIF1α-PPARγ pathway.; Authors - Chang-Chun Song
human	WB	Int J Mol Sci; Sustained Hypoxia Suppresses Joint Destruction in a Rat Model of Rheumatoid Arthritis via Negative Feedback of Hypoxia Inducible Factor-1α.; Authors - Kenta Kaihara
mouse	WB	Biomed Pharmacother; A novel PHD2 inhibitor acteoside from Cistanche tubulosa induces skeletal muscle mitophagy to improve cancer-related fatigue.; Authors - Shilei Zhang
human	WB	Sci Rep; Mitochondrial MUL1 E3 ubiquitin ligase regulates Hypoxia Inducible Factor (HIF-1α) and metabolic reprogramming by modulating the UBXN7 cofactor protein.; Authors - Lucia Cilenti