EGFR (C-terminal) Polyclonal antibody

EGFR (C-terminal) Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

25

Reviews (1)

Cat no : 51071-2-AP

Synonyms

EGFR, EGFR (C-terminal), ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1

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Validation Data Gallery

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  WB analysis of A431 using 51071-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 51071-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 51071-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 51071-2-AP

  IP result of anti-EGFR (C-terminal) (IP:51071-2-AP, 4ug; Detection:51071-2-AP 1:30000) with A431 cells lysate 1200 ug.

• 

  IF Staining of A431 using 51071-2-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR (C-terminal) antibody (51071-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, L02 cells
• Positive IP detected in: A431 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 25 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 2 publications below

Product Information

51071-2-AP targets EGFR (C-terminal) in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 1210 aa, 134 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC094761
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• RRID: AB_2096449
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. The antibody is specific to isoform1.

Publications

Species	Application	Title
human	WB	Cell Metab; Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity; Authors - Zilong Zhang
human	WB	Mol Ther; Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase; Authors - Yang Wang
mouse	WB	Cell Chem Biol; Discovery of a covalent inhibitor of heat shock protein 90 with antitumor activity that blocks the co-chaperone binding via C-terminal modification.; Authors - Li Li
human	WB	Front Pharmacol; Exploring the pharmacological mechanisms of icaritin against nasopharyngeal carcinoma via network pharmacology and experimental validation; Authors - Minglu Liu
human	WB,IF	Int J Mol Sci; JAC4 Inhibits EGFR-Driven Lung Adenocarcinoma Growth and Metastasis through CTBP1-Mediated JWA/AMPK/NEDD4L/EGFR Axis; Authors - Kun Ding
human	WB,CoIP	Int J Mol Sci; Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer; Authors - Hsien-Neng Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anna (Verified Customer) (02-27-2024)

Very good working antibodies in wetsren blot method

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: GC-1 spg cell culture

EGFR (C-terminal) Antibody Western Blot validation (1:2000 dilution) in GC-1 spg cell culture (Cat no:51071-2-AP)