EGFR Polyclonal antibody

EGFR Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 30139-1-AP

Synonyms

EGFR, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1

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Validation Data Gallery

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  WB analysis using 30139-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 30139-1-AP (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 30139-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 30139-1-AP (EGFR antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 30139-1-AP

  IP result of anti-EGFR (IP:30139-1-AP, 4ug; Detection:30139-1-AP 1:4000) with HeLa cells lysate 1520 ug.

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  IF Staining of A431 using 30139-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR antibody (30139-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30139-1-AP targets EGFR in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 134kd
• Observed Molecular Weight: 145-165 kDa
• GenBank Accession Number: NM_005228.5
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGFR is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer.