EGFL7 Polyclonal antibody

EGFL7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26661-1-AP

Synonyms

Multiple EGF-like domains protein 7, MEGF7, Epidermal growth factor-like protein 7, EGF-like protein 7, EGF like protein 7

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Validation Data Gallery

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  WB analysis using 26661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26661-1-AP (EGFL7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 26661-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26661-1-AP (EGFL7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 26661-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26661-1-AP (EGFL7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells, K-562 cells, RKO cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26661-1-AP targets EGFL7 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EGFL7 fusion protein Ag24693
• Full Name: EGF-like-domain, multiple 7
• Calculated Molecular Weight: 273 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC012377
• Gene Symbol: EGFL7
• Gene ID (NCBI): 51162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epidermal growth factor-like protein 7 (EGFL7, also known as MEGF7, ZNEU1, and VE-STATIN) is a proangiogenic factor that has been widely described as having a vital role in tubulogenesis and regulation of angiogenesis, mainly during embryogenesis organogenesis (PMID: 22160377; 15162510). It also can reduce central nervous system inflammation and may play a role in tumorigenesis (PMID: 29483510; 37490307).