EGF Polyclonal antibody

EGF Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 27141-1-AP

Synonyms

EGF, HOMG4, Pro epidermal growth factor, URG, Urogastrone

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Validation Data Gallery

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  IHC staining of human skin using 27141-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 27141-1-AP (EGF antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 27141-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 27141-1-AP (EGF antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 27141-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 27141-1-AP (EGF antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 27141-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 27141-1-AP (EGF antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

27141-1-AP targets EGF in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EGF fusion protein Ag25997
• Full Name: epidermal growth factor (beta-urogastrone)
• Calculated Molecular Weight: 1207 aa, 134 kDa
• GenBank Accession Number: BC093731
• Gene Symbol: EGF
• Gene ID (NCBI): 1950
• RRID: AB_2880771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epidermal growth factor (EGF) is a small 6 kD polypeptide and has six conserved cysteine residues that form three intramolecular disulfide bonds. Human EGF is synthesized as transmembrane precursor proteins (1207 amino acids), which are proteolytically cleaved to generate the 54 amino acid mature EGF.

Publications

Species	Application	Title
human	WB,IHC	Elife; METTL3 promotes homologous recombination repair and modulates chemotherapeutic response in breast cancer by regulating the EGF/RAD51 axis.; Authors - Enjie Li
		Genes (Basel); Evolutionary and Association Analysis of Buffalo FABP Family Genes Reveal Their Potential Role in Milk Performance.; Authors - Tingzhu Ye
human	WB	J Ethnopharmacol; Proteomics analysis of tumor exosomes reveals vital pathways of Jinfukang inhibiting circulating tumor cells metastasis in lung cancer.; Authors - Zu-Jun Que
human	IHC	Int J Exp Pathol; Clinicopathological features and immunophenotype of Silva pattern system in endocervical adenocarcinoma; Authors - Chao Zeng
human	WB	Evid Based Complement Alternat Med; Evodiamine Inhibits Gastric Cancer Cell Proliferation via PTEN-Mediated EGF/PI3K Signaling Pathway.; Authors - Ruichuang Yang
human	WB	ACS Appl Mater Interfaces; Paper/PMMA Hybrid 3D Cell Culture Microfluidic Platform for the Study of Cellular Crosstalk; Authors - Kin Fong Lei