EFTUD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

EFTUD2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA

Conjugate

Unconjugated

Publications

17

Cat no : 10208-1-AP

Synonyms

Snu114, SNRP116, KIAA0031, hSNU114, Elongation factor Tu GTP-binding domain-containing protein 2

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Validation Data Gallery

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  WB analysis of HeLa using 10208-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10208-1-AP (EFTUD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10208-1-AP

  WB result of EFTUD2 antibody (10208-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EFTUD2 transfected HeLa cells.

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  WB analysis of HeLa using 10208-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10208-1-AP (EFTUD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10208-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10208-1-AP (EFTUD2 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10208-1-AP

  IP result of anti-EFTUD2 (IP:10208-1-AP, 3ug; Detection:10208-1-AP 1:1000) with HeLa cells lysate 4650ug.

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  IP experiment using 10208-1-AP

  IP result of EFTUD2 (10208-1-AP for IP and Detection) with HeLa cell lysate.

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  IF Staining of HepG2 using 10208-1-AP

  Immunofluorescent analysis of HepG2 cells, using EFTUD2 antibody 10208-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

10208-1-AP targets EFTUD2 in WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EFTUD2 fusion protein Ag0279
• Full Name: elongation factor Tu GTP binding domain containing 2
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC002360
• Gene Symbol: EFTUD2
• Gene ID (NCBI): 9343
• RRID: AB_2095834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EFTUD2 encodes the 116 kDa U5 small nuclear ribonecleoprotein (snRNP) component, also named SNU114, it has GTP binding activity and is required for pre-mRNA splicing. Evolutionarily conserved human snRNP protein (U5-116kDa) is homologous to the ribosomal elongation factor EF-2 (ribosomal translocase). Defects in EFTUD2 are the cause of mandibulofacial dysostosis with microcephaly (MFDM). So far, acetylation and phosphorylation sites of EFTUD2 have been identified. Catolog#10208-1-AP is a rabbit polyclonal antibody raised against the N-terminal of human EFTUD2.

Publications

Species	Application	Title
mouse	WB	Nat Immunol; Syndromic immune disorder caused by a viable hypomorphic allele of spliceosome component Snrnp40.; Authors - Duanwu Zhang
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou
mouse	WB	FASEB J; Reduced prostaglandin I2 signaling in Arid5b-/- primary skeletal muscle cells attenuates myogenesis.; Authors - Jennifer Murray
human	WB	Mol Cell Biol; A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis.; Authors - Berg Michael G MG
human	IF	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao
human	PLA,CoIP	iScience; Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells; Authors - Nam-Kyung Yu