EFS Polyclonal antibody

EFS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20538-1-AP

Synonyms

CAS3, CASS3, EFS, EFS1, EFS2, HEFS, SIN

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Validation Data Gallery

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  WB analysis using 20538-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20538-1-AP (EFS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 20538-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20538-1-AP (EFS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 20538-1-AP

  IP result of anti-EFS (IP:20538-1-AP, 4ug; Detection:20538-1-AP 1:300) with mouse thymus tissue lysate 3200ug.

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  IP experiment of mouse thymus using 20538-1-AP

  IP result of anti-EFS (IP:20538-1-AP, 4ug; Detection:20538-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

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  IHC staining of human spleen using 20538-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 20538-1-AP (EFS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 20538-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 20538-1-AP (EFS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20538-1-AP

  Immunofluorescent analysis of HepG2 cells, using EFS antibody 20538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse thymus tissue, human placenta tissue
• Positive IP detected in: mouse thymus tissue
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20538-1-AP targets EFS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EFS fusion protein Ag14565
• Full Name: embryonal Fyn-associated substrate
• Calculated Molecular Weight: 561 aa, 59 kDa
• Observed Molecular Weight: 80-90 kDa, 67-70 kDa
• GenBank Accession Number: BC034246
• Gene Symbol: EFS
• Gene ID (NCBI): 10278
• RRID: AB_10695758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Genet; LYPD3, a New Biomarker and Therapeutic Target for Acute Myelogenous Leukemia.; Authors - Tingting Hu