mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20538-1-AP (EFS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20538-1-AP (EFS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 20538-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 20538-1-AP (EFS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
human placenta tissue were subjected to SDS PAGE followed by western blot with 20538-1-AP (EFS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse thymus using 20538-1-AP
IP result of anti-EFS (IP:20538-1-AP, 4ug; Detection:20538-1-AP 1:300) with mouse thymus tissue lysate 3200ug.
IP result of anti-EFS (IP:20538-1-AP, 4ug; Detection:20538-1-AP 1:300) with mouse thymus tissue lysate 2800ug.
IHC staining of human spleen using 20538-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 20538-1-AP (EFS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 20538-1-AP (EFS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human spleen using 20538-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 20538-1-AP (EFS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 20538-1-AP (EFS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 20538-1-AP
Immunofluorescent analysis of HepG2 cells, using EFS antibody 20538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:300-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20538-1-AP (EFS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 20538-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 20538-1-AP (EFS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human spleen using 20538-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 20538-1-AP (EFS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human spleen using 20538-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 20538-1-AP (EFS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse thymus using 20538-1-AP
IP result of anti-EFS (IP:20538-1-AP, 4ug; Detection:20538-1-AP 1:300) with mouse thymus tissue lysate 3200ug.
IP experiment of mouse thymus using 20538-1-AP
IP result of anti-EFS (IP:20538-1-AP, 4ug; Detection:20538-1-AP 1:300) with mouse thymus tissue lysate 2800ug.
IF/ICC Figures
IF Staining of HepG2 using 20538-1-AP
Immunofluorescent analysis of HepG2 cells, using EFS antibody 20538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
EFS Polyclonal antibody
Catalog Number
20538-1-AP
Citations
2
Dilutions
WB : 1:300-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
