EFCAB5 Polyclonal antibody

EFCAB5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24296-1-AP

Synonyms

EF-hand calcium-binding domain-containing protein 5

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Validation Data Gallery

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  WB analysis of HepG2 using 24296-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24296-1-AP (EFCAB5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 24296-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 24296-1-AP (EFCAB5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 24296-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50.

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  IHC staining of human liver cancer using 24296-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50.

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  IHC staining of human colon using 24296-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24296-1-AP (EFCAB5 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 24296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 24296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24296-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 24296-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 24296-1-AP

  Immunofluorescent analysis of HepG2 cells using 24296-1-AP (EFCAB5 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells
• Positive IHC detected in: human liver cancer tissue, human colon tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24296-1-AP targets EFCAB5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EFCAB5 fusion protein Ag19274
• Full Name: EF-hand calcium binding domain 5
• Calculated Molecular Weight: 1503 aa, 173 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC137519
• Gene Symbol: EFCAB5
• Gene ID (NCBI): 374786
• RRID: AB_2879479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.