CRACR2A Polyclonal antibody

• Featured Product
• KD/KO Validated

CRACR2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15206-1-AP

Synonyms

EFCAB4B, RAB46, EF-hand calcium-binding domain-containing protein 4B, CRAC channel regulator 2A, Calcium release-activated channel regulator 2A

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Validation Data Gallery

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  WB analysis of Jurkat using 15206-1-AP

  WB result of CRACR2A antibody (15206-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRACR2A transfected Jurkat cells.

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  WB analysis of Jurkat using 15206-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15206-1-AP (CRACR2A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 15206-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 15206-1-AP (CRACR2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15206-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15206-1-AP (CRACR2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15206-1-AP

  IP result of anti-CRACR2A (IP:15206-1-AP, 4ug; Detection:15206-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of human tonsillitis using 15206-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15206-1-AP (CRACR2A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15206-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15206-1-AP (CRACR2A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 15206-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 15206-1-AP (CRACR2A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, Daudi cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

15206-1-AP targets CRACR2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRACR2A fusion protein Ag7358
• Full Name: EF-hand calcium binding domain 4B
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa, 80-90 kDa
• GenBank Accession Number: BC004524
• Gene Symbol: CRACR2A
• Gene ID (NCBI): 84766
• RRID: AB_2246413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.

Publications

Species	Application	Title
human	WB,IP	Elife; Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder.; Authors - Beibei Wu
mouse	WB	J Immunol; CRACR2A-Mediated TCR Signaling Promotes Local Effector Th1 and Th17 Responses.; Authors - Jin Seok Woo
human	IP,IF,WB	Sci Rep; Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells.; Authors - Lucia Pedicini
human	WB	Biochem Biophys Res Commun; A long variant of CRACR2A that belongs to the Rab GTPase protein family.; Authors - Lesley A Wilson ; KD Validated
human	WB,IF	J Cell Biol; Rab46 integrates Ca2+ and histamine signaling to regulate selective cargo release from Weibel-Palade bodies.; Authors - Katarina T Miteva ; KD Validated
mouse	IF	Discov Immunol; Rab46: a novel player in mast cell function; Authors - Lucia Pedicini

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