eEF2K Polyclonal antibody

• Featured Product
• KD/KO Validated

eEF2K Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13510-1-AP

Synonyms

eEF-2K, eEF-2 kinase, eEF 2K, eEF 2 kinase, EC:2.7.11.20

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 13510-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13510-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13510-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 13510-1-AP

  IP result of anti-eEF2K (IP:13510-1-AP, 4ug; Detection:13510-1-AP 1:500) with PC-3 cells lysate 3200ug.

• 

  IF Staining of PC-3 using 13510-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13510-1-AP (EEF2K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: PC-3 cells, Jurkat cells, HEK-293 cells
• Positive IP detected in: PC-3 cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

13510-1-AP targets eEF2K in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: eEF2K fusion protein Ag4432
• Full Name: eukaryotic elongation factor-2 kinase
• Calculated Molecular Weight: 725 aa, 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC032665
• Gene Symbol: EEF2K
• Gene ID (NCBI): 29904
• RRID: AB_2277761
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Lett; eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells.; Authors - Cheng Ji
human	WB	Oncol Lett; AMPK decreases ERK1/2 activity and cancer cell sensitivity to nutrition deprivation by mediating a positive feedback loop involving eEF2K.; Authors - Shujuan Tong
rat	WB,IP,CoIP	Cells; SIRT1 Protects the Heart from ER Stress-Induced Injury by Promoting eEF2K/eEF2-Dependent Autophagy.; Authors - Julie Pires Da Silva ; KD Validated
human	WB	Med Oncol; A drug repurposing study identifies novel FOXM1 inhibitors with in vitro activity against breast cancer cells; Authors - Khaled A N Abusharkh
		J Mol Med (Berl); FOXM1 plays a role in autophagy by transcriptionally regulating Beclin-1 and LC3 genes in human triple-negative breast cancer cells.; Authors - Zuhal Hamurcu