EEF2 Polyclonal antibody, PBS Only

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  WB analysis using 20107-1-AP (same clone as 20107-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 20107-1-AP (same clone as 20107-1-PBS)

  WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HEK-293 cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

  WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HeLa cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 20107-1-AP (same clone as 20107-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

  IP result of anti-EEF2 (IP:20107-1-AP, 3ug; Detection:20107-1-AP 1:500) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 20107-1-AP (same clone as 20107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20107-1-AP (EEF2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20107-1-AP (same clone as 20107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 20107-1-AP (same clone as 20107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20107-1-AP (same clone as 20107-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (20107-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human EEF2 (20107-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

EEF2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 20107-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

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Product Information

20107-1-PBS targets EEF2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EEF2 fusion protein Ag13826
• Full Name: eukaryotic translation elongation factor 2
• Calculated Molecular Weight: 858 aa, 95 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC126259
• Gene Symbol: EEF2
• Gene ID (NCBI): 1938
• RRID: AB_10950401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13639
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.