WB analysis using 20107-1-AP (same clone as 20107-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 20107-1-AP (same clone as 20107-1-PBS)
WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HEK-293 cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HEK-293 cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 20107-1-AP (same clone as 20107-1-PBS)
WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HeLa cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HeLa cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 20107-1-AP (same clone as 20107-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)
IP result of anti-EEF2 (IP:20107-1-AP, 3ug; Detection:20107-1-AP 1:500) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IP result of anti-EEF2 (IP:20107-1-AP, 3ug; Detection:20107-1-AP 1:500) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 20107-1-AP (same clone as 20107-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20107-1-AP (EEF2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20107-1-AP (EEF2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 20107-1-AP (same clone as 20107-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 20107-1-AP (same clone as 20107-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 20107-1-AP (same clone as 20107-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (20107-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (20107-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human EEF2 (20107-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human EEF2 (20107-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
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WB analysis using 20107-1-AP (same clone as 20107-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 20107-1-AP (same clone as 20107-1-PBS)
WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HEK-293 cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 20107-1-AP (same clone as 20107-1-PBS)
WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HeLa cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 20107-1-AP (same clone as 20107-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IHC Figures
IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 20107-1-AP (same clone as 20107-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20107-1-AP (EEF2 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 20107-1-AP (same clone as 20107-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 20107-1-AP (same clone as 20107-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IP Figures
IP experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)
IP result of anti-EEF2 (IP:20107-1-AP, 3ug; Detection:20107-1-AP 1:500) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of HepG2 using 20107-1-AP (same clone as 20107-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (20107-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
FC (INTRA) Figures
FC experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human EEF2 (20107-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
EEF2 Polyclonal antibody
Catalog Number
20107-1-PBS
Citations
-
Dilutions
Applications
WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
