EEF1D Monoclonal antibody

• Featured Product
• KD/KO Validated

EEF1D Monoclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3G4C4

Cat no : 60085-2-Ig

Synonyms

Antigen NY CO 4, EEF1D, EF 1 delta, EF 1D, EF1D, Elongation factor 1 delta, FP1047

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Validation Data Gallery

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  WB analysis of MCF-7 using 60085-2-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60085-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 60085-2-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60085-2-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60085-2-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 60085-2-Ig

  IP result of anti-EEF1D (IP:60085-2-Ig, 5ug; Detection:60085-2-Ig 1:1000) with MCF-7 cells lysate 3200ug.

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  IHC staining of human pancreas cancer using 60085-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50.

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  IHC staining of human pancreas cancer using 60085-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: transfection MCF-7 cells, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

60085-2-Ig targets EEF1D in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EEF1D fusion protein Ag0983
• Full Name: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC007847
• Gene Symbol: EEF1D
• Gene ID (NCBI): 1936
• RRID: AB_2096994
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

Publications

Species	Application	Title
human	WB,IF	J Virol; Eukaryotic translation elongation factor 1 delta inhibits the nuclear import of nucleoprotein and PA-PB1 heterodimer of influenza A virus.; Authors - Qingxia Gao ; KO Validated