EEF1D Monoclonal antibody

• Featured Product
• KD/KO Validated

EEF1D Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

3B1B11

Cat no : 60085-1-Ig

Synonyms

EF-1-delta, EF1D, EF 1D, EF 1 delta, Antigen NY-CO-4

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Validation Data Gallery

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  WB analysis of Jurkat using 60085-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 60085-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60085-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 60085-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60085-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 60085-1-Ig

  IP result of anti-EEF1D (IP:60085-1-Ig, 5ug; Detection:60085-1-Ig 1:1000) with MCF-7 cells lysate 3200ug.

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  IHC staining of human pancreas cancer using 60085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60085-1-Ig (EEF1D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 60085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 60085-1-Ig (EEF1D Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 60085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 60085-1-Ig (EEF1D Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 60085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60085-1-Ig (EEF1D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 60085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60085-1-Ig(EEF1D antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 60085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60085-1-Ig(EEF1D antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 60085-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1D antibody (60085-1-Ig, Clone: 3B1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  IF Staining of HeLa using 60085-1-Ig

  Immunofluorescent analysis of Hela cells, using EEF1D antibody 60085-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human pancreas cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

60085-1-Ig targets EEF1D in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EEF1D fusion protein Ag0983
• Full Name: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC007847
• Gene Symbol: EEF1D
• Gene ID (NCBI): 1936
• RRID: AB_2096996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

Publications

Species	Application	Title
human	WB	Cell Chem Biol; A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.; Authors - Yingjie Hou
mouse	WB	Free Radic Biol Med; Proteomic analysis of the glutathione-deficient LEGSKO mouse lens reveals activation of EMT signaling, loss of lens specific markers, and changes in stress response proteins.; Authors - Jeremy A Whitson
human	WB	Front Microbiol; Strain-Specific Contribution of Eukaryotic Elongation Factor 1 Gamma to the Translation of Influenza A Virus Proteins.; Authors - Shuhei Sammaibashi
human	WB,IF	J Virol; Eukaryotic translation elongation factor 1 delta inhibits the nuclear import of nucleoprotein and PA-PB1 heterodimer of influenza A virus.; Authors - Qingxia Gao ; KO Validated
human	IP	STAR Protoc; Protocol for identification and validation of 2'3'-cGAMP-binding proteins by photoaffinity probes.; Authors - Yingjie Hou