EED Monoclonal antibody, PBS Only

EED Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D11D4

Cat no : 67756-1-PBS

Synonyms

EED, embryonic ectoderm development, HEED, Polycomb protein EED, WAIT 1, WAIT1

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Validation Data Gallery

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  WB analysis using 67756-1-Ig (same clone as 67756-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67756-1-Ig (EED antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67756-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67756-1-Ig (same clone as 67756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67756-1-Ig (EED antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67756-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67756-1-Ig (same clone as 67756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67756-1-Ig (EED antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67756-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 67756-1-Ig (same clone as 67756-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EED antibody (67756-1-Ig, Clone: 1D11D4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67756-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67756-1-Ig (same clone as 67756-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using EED antibody (67756-1-Ig, Clone: 1D11D4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67756-1-PBS in a different storage buffer formulation.

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Product Information

67756-1-PBS targets EED in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: EED fusion protein Ag10369
• Full Name: embryonic ectoderm development
• Calculated Molecular Weight: 466 aa, 53 kDa
• Observed Molecular Weight: 50/53/46 kDa.
• GenBank Accession Number: BC068995
• Gene Symbol: EED
• Gene ID (NCBI): 8726
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.