EDNRB Recombinant antibody

EDNRB Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1O2

Cat no : 82818-2-RR

Synonyms

ET BR, ET B, endothelin receptor type B, Endothelin receptor non-selective type, Endothelin B receptor

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Validation Data Gallery

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  WB analysis using 82818-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82818-2-RR (EDNRB antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  FC experiment of Raji using 82818-2-RR

  1x10^6 Raji cells were intracellularly stained with 0.25 ug EDNRB Recombinant antibody (82818-2-RR, Clone:1O2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, mouse cerebellum tissue, rat brain tissue
• Positive FC (Intra) detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82818-2-RR targets EDNRB in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: endothelin receptor type B
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_000115
• Gene Symbol: EDNRB
• Gene ID (NCBI): 1910
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EDNRB (endothelin receptor type B) is a G-protein coupled heptahelical endothelin-1 (EDN1) receptor. EDN1, a known mediator of human myometrial contraction in vitro, belongs to a family of three 21-amino acid isopeptides (PMID:18497345). EDNRB is a 442-residue protein expressed post-natally in many tissues, particularly in the myenteric plexus, mucosal layer, ganglia, and blood vessels of the submucosa of the colon (PMID:11434563). Moreover, Both the EDNRA and EDNRB are expressed in vessels and can be bound by EDN1, but while EDNRA mediates a constrictive effect, EDNRB is involved in vasorelaxation and functions as a clearance receptor that scavenges circulating EDN1 (PMID:30171849).