EDAR Polyclonal antibody

EDAR Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18032-1-AP

Synonyms

DL, Downless homolog, Ectodermal dysplasia receptor, ectodysplasin A receptor, ED1R, ED3, ED5, EDA A1 receptor, EDA A1R, EDA1R, EDA3, EDAR, HRM1



Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, A431 cells, MCF-7 cells
Positive IP detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18032-1-AP targets EDAR in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EDAR fusion protein Ag12601
Full Name ectodysplasin A receptor
Calculated Molecular Weight 448 aa, 49 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC093872
Gene Symbol EDAR
Gene ID (NCBI) 10913
RRIDAB_10596932
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EDAR, also named as DL, is a receptor for EDA isoform A1, but not for EDA isoform A2. It mediates the activation of NF-kappa-B and JNK. EDAR may promote caspase-independent cell death. It is expressed in adjacent or partially overlapping layers in the developing human skin with MW 48 kDa.

Protocols

Product Specific Protocols
WB protocol for EDAR antibody 18032-1-APDownload protocol
IP protocol for EDAR antibody 18032-1-APDownload protocol
FC protocol for EDAR antibody 18032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Development

Involvement of Wnt, Eda and Shh at defined stages of sweat gland development.

Authors - Chang-Yi Cui
mouseWB

J Pharm Pharmacol

Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway.

Authors - Zhong-San Zeng
mouseIF,IHC

J Histochem Cytochem

Spatial and Temporal Expression of Ectodysplasin-A Signaling Pathway Members During Mandibular Condylar Development in Postnatal Mice

Authors - Ludan Xing
mouseWB

J Invest Dermatol

ASH2L mediates epidermal differentiation and hair follicle morphogenesis via H3K4me3 modification

Authors - Qirui Wang