EDA Polyclonal antibody

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  WB analysis of mouse liver using 25892-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25892-1-AP (EDA Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 25892-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25892-1-AP (EDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 25892-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25892-1-AP (EDA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EDA Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25892-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Ectodermal dysplasia protein, ectodysplasin A, ED1, ED1 A1, ED1 A2, EDA, EDA protein, EDA1, EDA2, HED, XHED, XLHED

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

25892-1-AP targets EDA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EDA fusion protein Ag23154
• Full Name: ectodysplasin A
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC126143
• Gene Symbol: EDA
• Gene ID (NCBI): 1896
• RRID: AB_2880286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92838
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Genes (Basel); EDA Mutations Causing X-Linked Recessive Oligodontia with Variable Expression; Authors - Ye Ji Lee
human	IHC	Heliyon; Identification of cancer-associated fibroblast-related Ectodysplasin-A as a novel indicator for prognosis and immune response in gastric cancer; Authors - Ya Zhang
human	WB	Clin Cosmet Investig Dermatol; A Novel Ectodysplasin a Gene mutation of X-Linked Hypohidrotic Ectodermal Dysplasia; Authors - Yuan Zhuang