ECSIT Recombinant antibody, PBS Only (Detector)

ECSIT Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240223A7

Cat no : 83295-1-PBS

Synonyms

SITPEC, Protein SITPEC, ECSIT homolog (Drosophila), 240223A7

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Validation Data Gallery

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  WB analysis using 83295-1-RR (same clone as 83295-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83295-1-RR (ECSIT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 83295-1-RR (same clone as 83295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83295-1-RR (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 83295-1-RR (same clone as 83295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83295-1-RR (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83295-1-RR (same clone as 83295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83295-1-RR (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83295-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83295-1-RR (same clone as 83295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83295-1-RR (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83295-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00315-1

  Cytometric bead array standard curve of MP00315-1, ECSIT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83295-4-PBS. Detection antibody: 83295-1-PBS. Standard: Ag26898. Range: 3.13-100 ng/mL.

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  Affinity and Kinetic Characterization of 83295-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83295-1-RR against Human ECSIT were performed. The affinity constant is below 1 pM.

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Product Information

83295-1-PBS targets ECSIT as part of a matched antibody pair:

MP00315-1: 83295-4-PBS capture and 83295-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ECSIT fusion protein Ag26898
• Full Name: ECSIT homolog (Drosophila)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC008279
• Gene Symbol: ECSIT
• Gene ID (NCBI): 51295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ECSIT is a cytosolic adaptor protein associated with the toll-like receptor pathway. It has a distinct N-terminal mitochondrial targeting sequence, pentatricopeptide repeat motif, and a C-terminal pleckstrin homology domain. ECSIT regulates many biological processes like embryonic development, inflammation, cardiac function, and assembly of mitochondrial complex I. In macrophages, the activation of TLRs by inflammatory signals promotes the translocation of TRAF6 to mitochondria where it engages ECSIT to stimulate the production of mitochondrial reactive oxygen species (ROS).