ECSIT Polyclonal antibody

ECSIT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27548-1-AP

Synonyms

SITPEC, Protein SITPEC, ECSIT homolog (Drosophila)

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Validation Data Gallery

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  WB analysis using 27548-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27548-1-AP (ECSIT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27548-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27548-1-AP (ECSIT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27548-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27548-1-AP (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 27548-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27548-1-AP (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells, mouse kidney tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27548-1-AP targets ECSIT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ECSIT fusion protein Ag26877
• Full Name: ECSIT homolog (Drosophila)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC008279
• Gene Symbol: ECSIT
• Gene ID (NCBI): 51295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ECSIT is a cytosolic adaptor protein associated with the toll-like receptor pathway. It has a distinct N-terminal mitochondrial targeting sequence, pentatricopeptide repeat motif, and a C-terminal pleckstrin homology domain. ECSIT regulates many biological processes like embryonic development, inflammation, cardiac function, and assembly of mitochondrial complex I. In macrophages, the activation of TLRs by inflammatory signals promotes the translocation of TRAF6 to mitochondria where it engages ECSIT to stimulate the production of mitochondrial reactive oxygen species (ROS).