ECM1 Monoclonal antibody, PBS Only (Capture)

ECM1 Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D1A3

Cat no : 68472-1-PBS

Synonyms

Secretory component p85, extracellular matrix protein 1, 2D1A3

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Validation Data Gallery

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  WB analysis using 68472-1-Ig (same clone as 68472-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 68472-1-Ig (same clone as 68472-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 68472-1-Ig (same clone as 68472-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 68472-1-Ig (same clone as 68472-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 68472-1-Ig (same clone as 68472-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50074-1

  Sandwich ELISA standard curve of MP50074-1, ECM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68472-1-PBS. Detection antibody: 66023-1-PBS. Standard: Eg0533. Range: 0.156-10 ng/mL.

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  IHC staining of human skin cancer using 68472-1-Ig (same clone as 68472-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68472-1-Ig (ECM1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

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Product Information

68472-1-PBS targets ECM1 as part of a matched antibody pair:

MP50074-1: 68472-1-PBS capture and 66023-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: extracellular matrix protein 1
• Calculated Molecular Weight: 540 aa, 61 kDa
• Observed Molecular Weight: 60-80 kDa
• GenBank Accession Number: BC023505
• Gene Symbol: ECM1
• Gene ID (NCBI): 1893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ECM1 (extracellular matrix protein 1), also known as URBWD. It is located in secreted, extracellular space, and extracellular matrix, which is mainly expressed in esophagus and gall bladder. The gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. The calculated molecular weight of ECM1 is 60 kDa, and this antibody can recognize the 63 kDa isoform of target, moreover, this protein may have glycosylation modification.