ECH1 Monoclonal antibody, PBS Only

ECH1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C9G5

Cat no : 68139-1-PBS

Synonyms

ECH1, HPXEL

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Validation Data Gallery

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  WB analysis using 68139-1-Ig (same clone as 68139-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68139-1-Ig (ECH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 68139-1-Ig (same clone as 68139-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ECH1 antibody (68139-1-Ig, Clone: 1C9G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68139-1-PBS targets ECH1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ECH1 fusion protein Ag32661
• Full Name: enoyl Coenzyme A hydratase 1, peroxisomal
• Calculated Molecular Weight: 328 aa, 36 kDa
• Observed Molecular Weight: 33-36 kDa
• GenBank Accession Number: BC017408
• Gene Symbol: ECH1
• Gene ID (NCBI): 1891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.