ECH1 Polyclonal antibody
ECH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 11385-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue, HepG2 cells, Jurkat cells, K-562 cells, L02 cells, mouse heart tissue |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 6 publications below |
Product Information
11385-1-AP targets ECH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ECH1 fusion protein Ag1931 |
Full Name | enoyl Coenzyme A hydratase 1, peroxisomal |
Calculated Molecular Weight | 328 aa, 36 kDa |
Observed Molecular Weight | 30-36 kDa |
GenBank Accession Number | BC017408 |
Gene Symbol | ECH1 |
Gene ID (NCBI) | 1891 |
RRID | AB_2277810 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Enoyl coenzyme A hydratase 1 (ECH1), is first recog-nized as a key component in mitochondrial fatty acid β-oxidation, and subsequent studies have demonstrated that it regulates multiple pathophysiological processes (PMID:33813878). The MW of ECH1 is 36 kDa,and can be detected as 30-36 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for ECH1 antibody 11385-1-AP | Download protocol |
IHC protocol for ECH1 antibody 11385-1-AP | Download protocol |
IF protocol for ECH1 antibody 11385-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity | ||
J Clin Invest Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice. | ||
Hepatology High fat and high sucrose (western) diet induce steatohepatitis that is dependent on fructokinase. | ||
Front Cell Dev Biol Single-Cell Transcriptomics Reveals Splicing Features of Adult Neural Stem Cells in the Subventricular Zone. | ||
Eur J Immunol YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice. | ||
Cell Death Differ C-IGF1R encoded by cIGF1R acts as a molecular switch to restrict mitophagy of drug-tolerant persister tumour cells in non-small cell lung cancer |