ECE1 Polyclonal antibody
ECE1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 27245-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HUVEC cells, MCF-7 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
27245-1-AP targets ECE1 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ECE1 fusion protein Ag26124 |
Full Name | endothelin converting enzyme 1 |
Observed Molecular Weight | 87-110 kDa |
GenBank Accession Number | BC126257 |
Gene Symbol | ECE1 |
Gene ID (NCBI) | 1889 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ECE1, Endothelin-converting enzyme 1, also known as ECE. It is located in cell membrane, and it has 4 isoforms. All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney (PMID: 9396733). The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. The calculated molecular weight of ECE1 is 87 kDa, as a result of glycosylation, the apparent molecular mass of ECE1 is approximately 87-110 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for ECE1 antibody 27245-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |