RCAS1 Polyclonal antibody

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• KD/KO Validated

RCAS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12255-1-AP

Synonyms

EBAG9, PDAF, Estrogen receptor-binding fragment-associated gene 9 protein, EBAG 9, EB9

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Validation Data Gallery

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  WB analysis using 12255-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12255-1-AP (RCAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12255-1-AP

  WB result of RCAS1 antibody (12255-1-AP, 1:10000) with si-control and si-RCAS1 transfected HEK293 cells.

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  IP experiment of HeLa using 12255-1-AP

  IP result of anti-RCAS1 (IP:12255-1-AP, 4ug; Detection:12255-1-AP 1:2000) with HeLa cells lysate 1720 ug.

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  IHC staining of human colon using 12255-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 12255-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 12255-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using RCAS1 antibody (12255-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse heart tissue, rat heart tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

12255-1-AP targets RCAS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RCAS1 fusion protein Ag2905
• Full Name: estrogen receptor binding site associated, antigen, 9
• Calculated Molecular Weight: 213 aa, 24 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC017729
• Gene Symbol: RCAS1
• Gene ID (NCBI): 9166
• RRID: AB_2246420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Estrogen receptor-binding fragment-associated antigen 9 (EBAG9) gene was identified as an estrogen-responsive gene. The gene product, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), is associated with aggressive characteristics and poor overall survival for 15 different human malignancies. The correlation between RCAS1 expression and several clinicopathological variables, including tumor size, clinical stage, invasion depth and lymph node metastasis highlights this molecule's clinical significance. Expression of RCAS1 in tumor cells plays an important role in evasion from host immune system resulting tumor progression, invasion and metastasis. Further exploration of RCAS1 biological function will facilitate development of novel therapeutic strategies that target RCAS1.

Publications

Species	Application	Title
rat	WB,IHC	J Ethnopharmacol; Exploring the inhibitory impact of Mongolian medicinal He-Zi-3 soup on mammary gland hyperplasia in rats induced by estrogen and progestogen; Authors - Chunlan Liang