EAF1 Polyclonal antibody

EAF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13787-1-AP

Synonyms

ELL-associated factor 1

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Validation Data Gallery

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  WB analysis of K-562 using 13787-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13787-1-AP (EAF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13787-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13787-1-AP (EAF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13787-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13787-1-AP (EAF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13787-1-AP

  IP result of anti-EAF1 (IP:13787-1-AP, 4ug; Detection:13787-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of SH-SY5Y using 13787-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13787-1-AP (EAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IP: See 1 publications below

Product Information

13787-1-AP targets EAF1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EAF1 fusion protein Ag4804
• Full Name: ELL associated factor 1
• Calculated Molecular Weight: 268 aa, 29 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC041329
• Gene Symbol: EAF1
• Gene ID (NCBI): 85403
• RRID: AB_2255402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. The tumor suppressor EAF2 is regulated by androgen signaling and associated with prostate cancer. Eaf factors have been revealed to modulate mesoderm and neural patterning by inhibiting canonical Wnt signaling, and high activity of canonical Wnt/β-catenin is revealed in eafs morphants (PMID: 23364330). Catalog#13787-1-AP is a rabbit polyclonal antibody raised against the full-length of human EAF1.

Publications

Species	Application	Title
human	WB	Mol Cell; HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP.; Authors - Bijan Sobhian
human	WB	Cell Death Differ; Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis.; Authors - Ping Liu
	WB	Biochim Biophys Acta; Transcriptional factors Eaf1/2 inhibit endoderm and mesoderm formation via suppressing TGF-β signaling.; Authors - Jing-Xia Liu
human	WB	Biochim Biophys Acta Gene Regul Mech; ELL-associated factors EAF1/2 negatively regulate HIV-1 transcription through inhibition of Super Elongation Complex formation.; Authors - Rongdiao Liu
mouse	WB,IP	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao