EAAT2 Monoclonal antibody, PBS Only

EAAT2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C8D10

Cat no : 67083-1-PBS

Synonyms

EAAT2, GLT 1, GLT1, SLC1A2

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Validation Data Gallery

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  WB analysis using 67083-1-Ig (same clone as 67083-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67083-1-Ig (SLC1A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67083-1-Ig (same clone as 67083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67083-1-Ig (same clone as 67083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67083-1-Ig (same clone as 67083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67083-1-Ig (same clone as 67083-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

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Product Information

67083-1-PBS targets EAAT2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EAAT2 fusion protein Ag19816
• Full Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2
• Calculated Molecular Weight: 574 aa, 62 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC132768
• Gene Symbol: EAAT2
• Gene ID (NCBI): 6506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EAAT2 (also known as GLT-1), is encoded by SLC1A2 and is a glutamate transporter which can clear the majority of extracellular glutamate in brain and prevent brain seizures.Three isoforms of EAAT2 exist due to the alternative splicing. In addition to the 65-70 kDa monomer form, 130-150 kDa dimer of EAAT2 can also be observed on SDS-PAGE.(PMID: 22114258)