E2F6 Polyclonal antibody

E2F6 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31464-1-AP

Synonyms

E2F-6, E2F 6

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Validation Data Gallery

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  WB analysis using 31464-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31464-1-AP (E2F6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 31464-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using E2F6 antibody (31464-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31464-1-AP targets E2F6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: E2F6 fusion protein Ag35112
• Full Name: E2F transcription factor 6
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC008348
• Gene Symbol: E2F6
• Gene ID (NCBI): 1876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

E2F6 is a member of the E2F family of TFs that control cell proliferation and cell fate. Cancer patients with high E2F6 expression in tumors, such as glioblastoma, breast cancer, and hepatocellular carcinoma, have poor prognosis.