E2F3 Polyclonal antibody

• Featured Product
• KD/KO Validated

E2F3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 27615-1-AP

Synonyms

E2F 3, E2F transcription factor 3, E2F3, KIAA0075, Transcription factor E2F3

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Validation Data Gallery

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  WB analysis of SGC-7901 using 27615-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 27615-1-AP (E2F3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 27615-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 27615-1-AP (E2F3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: SGC-7901 cells, SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 3 publications below

Product Information

27615-1-AP targets E2F3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: E2F3 fusion protein Ag26367
• Full Name: E2F transcription factor 3
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: NM_001243076
• Gene Symbol: E2F3
• Gene ID (NCBI): 1871
• RRID: AB_2880926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Mol Ther Nucleic Acids; miRNA in food simultaneously controls animal viral disease and human tumorigenesis.; Authors - Yalei Cui
human	WB	Mol Ther Nucleic Acids; Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer.; Authors - Yalei Cui
human	WB	Oncotarget; SNHG1 promotes cell proliferation by acting as a sponge of miR-145 in colorectal cancer.; Authors - Tian Tian
human	WB	Sci China Life Sci; rtcisE2F promotes the self-renewal and metastasis of liver tumor-initiating cells via N6-methyladenosine-dependent E2F3/E2F6 mRNA stability.; Authors - Zhenzhen Chen ; KO Validated
human	WB	Phytomedicine; Apigenin inhibits the growth of colorectal cancer through down-regulation of E2F1/3 by miRNA-215-5p.; Authors - Yuan Cheng
human	WB	Int J Mol Med; MicroRNA‑145‑5p inhibits osteosarcoma cell proliferation by targeting E2F transcription factor 3.; Authors - Hao Li