E2F3 Polyclonal antibody

E2F3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12334-1-AP

Synonyms

E2F3, Q96AR0

---

Validation Data Gallery

• 

  WB analysis of Y79 using 12334-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12334-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12334-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12334-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12334-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12334-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12334-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse liver using 12334-1-AP

  IP result of anti-E2F3 (IP:12334-1-AP, 4ug; Detection:12334-1-AP 1:500) with mouse liver tissue lysate 4000ug.

---

Tested Applications

• Positive WB detected in: Y79 cells, HEK-293 cells, HepG2 cells, mouse liver tissue
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

12334-1-AP targets E2F3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: E2F3 fusion protein Ag3039
• Full Name: E2F transcription factor 3
• Calculated Molecular Weight: 133 aa, 15 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC016847
• Gene Symbol: E2F3
• Gene ID (NCBI): 1871
• RRID: AB_10597554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

E2F transcription factors regulate gene expression in concert with the retinoblastoma tumor suppressor family. These transcriptional complexes are master regulators of cell cycle progression, control the expression of genes involved in DNA repair, G2/M checkpoint and differentiation [PMID:22580460]. E2f3 is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner [PMID:22960857].

Publications

Species	Application	Title
human	WB, IHC	Nat Commun; POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.; Authors - Boshi Wang
human	WB	Mol Med Rep; [Corrigendum] Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells.; Authors - Dacai Xu
human	WB	Mol Med Rep; Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells.; Authors - Dacai Xu
human,mouse	WB	Cancer Lett; PA28γ coordinates the cross-talk between cancer-associated fibroblasts and tumor cells to promote OSCC progression via HDAC1/E2F3/IGF2 signaling; Authors - Zaiye Li