E-cadherin Recombinant antibody, PBS Only

E-cadherin Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240159G8

Cat no : 80541-9-PBS

Synonyms

CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1

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Validation Data Gallery

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  WB analysis using 80541-9-RR (same clone as 80541-9-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80541-9-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80541-9-PBS in a different storage buffer formulation.

• 

  WB analysis using 80541-9-RR (same clone as 80541-9-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80541-9-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80541-9-PBS in a different storage buffer formulation.

• 

  WB analysis using 80541-9-RR (same clone as 80541-9-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80541-9-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80541-9-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 80541-9-RR (same clone as 80541-9-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80541-9-RR (E-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80541-9-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 80541-9-RR (same clone as 80541-9-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using E-cadherin antibody (80541-9-RR, Clone: 240159G8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 80541-9-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80541-9-RR

  Biolayer interferometry (BLl) kinetic assays of 80541-9-RR against Human E-cadherin were performed. The affinity constant is 0.213 nM.

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Product Information

The immunogen of 80541-9-PBS is E-cadherin Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: E-cadherin fusion protein Ag14973
• Full Name: cadherin 1, type 1, E-cadherin (epithelial)
• Calculated Molecular Weight: 882 aa, 97 kDa
• GenBank Accession Number: BC141838
• Gene Symbol: E-cadherin
• Gene ID (NCBI): 999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.