E-cadherin Recombinant antibody, PBS Only

E-cadherin Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240159A1

Cat no : 80541-7-PBS

Synonyms

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, Epithelial cadherin, LCAM, UVO, Uvomorulin

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Validation Data Gallery

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  WB analysis using 80541-7-RR (same clone as 80541-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80541-7-RR (E-cadherin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80541-7-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 80541-7-RR (same clone as 80541-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using E-cadherin antibody (80541-7-RR, Clone: 240159A1 ) at dilution of 1:160 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80541-7-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 80541-7-PBS is E-cadherin Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: E-cadherin fusion protein Ag14973
• Full Name: cadherin 1, type 1, E-cadherin (epithelial)
• Calculated Molecular Weight: 882 aa, 97 kDa
• GenBank Accession Number: BC141838
• Gene Symbol: CDH1
• Gene ID (NCBI): 999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.