E-cadherin Polyclonal antibody

E-cadherin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31515-1-AP

Synonyms

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, Epithelial cadherin, LCAM, UVO, Uvomorulin

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Validation Data Gallery

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  WB analysis using 31515-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31515-1-AP (E-cadherin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, T-47D cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31515-1-AP targets E-cadherin in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: cadherin 1, type 1, E-cadherin (epithelial)
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 120-125 kDa
• GenBank Accession Number: NM_004360.5
• Gene Symbol: E-cadherin
• Gene ID (NCBI): 999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also includes N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.