Mouse E-Cadherin Recombinant antibody, PBS Only (Detector)

E-Cadherin Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241282A8

Cat no : 84111-2-PBS

Synonyms

Um, L CAM, Ecad, Cdh1, cadherin 1

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Validation Data Gallery

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  WB analysis using 84111-2-RR (same clone as 84111-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84111-2-RR (E-Cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84111-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 84111-2-RR (same clone as 84111-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84111-2-RR (E-Cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 84111-2-RR (same clone as 84111-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84111-2-RR (E-Cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84111-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84111-2-RR (same clone as 84111-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using E-Cadherin antibody (84111-2-RR, Clone: 241282A8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84111-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01047-1

  Cytometric bead array standard curve of MP01047-1, MOUSE E-Cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84111-4-PBS. Detection antibody: 84111-2-PBS. Standard: Eg0975. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP01047-2

  Cytometric bead array standard curve of MP01047-2, MOUSE E-Cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84111-1-PBS. Detection antibody: 84111-2-PBS. Standard: Eg0975. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP01047-3

  Cytometric bead array standard curve of MP01047-3, MOUSE E-Cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84111-3-PBS. Detection antibody: 84111-2-PBS. Standard: Eg0975. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84111-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84111-2-RR against Mouse E-Cadherin were performed. The affinity constant is below 1 pM.

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Product Information

84111-2-PBS targets E-Cadherin as part of a matched antibody pair:

MP01047-1: 84111-4-PBS capture and 84111-2-PBS detection (validated in Cytometric bead array)

MP01047-2: 84111-1-PBS capture and 84111-2-PBS detection (validated in Cytometric bead array)

MP01047-3: 84111-3-PBS capture and 84111-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: cadherin 1
• Calculated Molecular Weight: 98kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: NM_009864.3
• Gene Symbol: E-cadherin
• Gene ID (NCBI): 12550
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+.