Dystrophin Recombinant antibody

Dystrophin Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240567A3

Cat no : 83609-5-RR

Synonyms

DMD, BMD, 240567A3

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Validation Data Gallery

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  WB analysis using 83609-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83609-5-RR (DMD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 83609-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83609-5-RR (Dystrophin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 83609-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 83609-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 83609-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 83609-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Dystrophin antibody (83609-5-RR, Clone: 240567A3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HepG2 using 83609-5-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Dystrophin Recombinant antibody (83609-5-RR, Clone:240567A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83609-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83609-5-RR against Human Dystrophin were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83609-5-RR is Dystrophin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Dystrophin fusion protein Ag3408
• Full Name: dystrophin
• Calculated Molecular Weight: 3685 aa, 427 kDa
• Observed Molecular Weight: 430 kDa, 70 kDa
• GenBank Accession Number: BC028720
• Gene Symbol: Dystrophin
• Gene ID (NCBI): 1756
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.