Drebrin Monoclonal antibody

Drebrin Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A12H1

Cat no : 67589-1-Ig

Synonyms

D0S117E, DBN1, DKFZp434D064, Drebrin, drebrin 1

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Validation Data Gallery

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  WB analysis using 67589-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67589-1-Ig (Drebrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67589-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67589-1-Ig (Drebrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67589-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67589-1-Ig (Drebrin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 67589-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Drebrin antibody (67589-1-Ig, Clone: 1A12H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue).

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Tested Applications

• Positive WB detected in: HeLa cells, pig brain tissue, HEK-293 cells, PC-12 cells, Neuro-2a cells, rat brain tissue, mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67589-1-Ig targets Drebrin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Drebrin fusion protein Ag22635
• Full Name: drebrin 1
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC007567
• Gene Symbol: Drebrin
• Gene ID (NCBI): 1627
• RRID: AB_2882797
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.