Drebrin Polyclonal antibody

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  WB analysis using 25770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25770-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 25770-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 25770-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25770-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 25770-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 25770-1-AP (Drebrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 25770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Drebrin antibody (25770-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 25770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 25770-1-AP (Drebrin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

Drebrin Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25770-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

D0S117E, DBN1, Drebrin, drebrin 1

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, SH-SY5Y cells, K-562 cells, Neuro-2a cells, Jurkat cells
• Positive IF/ICC detected in: MCF-7 cells, SH-SY5Y cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

25770-1-AP targets Drebrin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Drebrin fusion protein Ag22634
• Full Name: drebrin 1
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC007567
• Gene Symbol: Drebrin
• Gene ID (NCBI): 1627
• ENSEMBL Gene ID: ENSG00000113758
• RRID: AB_2880234
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16643
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

Publications

Species	Application	Title
human	IF	Int J Mol Sci; Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery; Authors - Paloma Alvarez-Suarez