Di/Tri-Methyl-Histone H3 (Lys4) Recombinant antibody, PBS Only

Di/Tri-Methyl-Histone H3 (Lys4) Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Dot Blot, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241942D11

Cat no : 84908-2-PBS

Synonyms

HIST1H3A, 241942D11, Histone-H3, HistoneH3, Histone H3

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Validation Data Gallery

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  WB analysis using 84908-2-RR (same clone as 84908-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84908-2-RR (same clone as 84908-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84908-2-RR (same clone as 84908-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84908-2-RR (same clone as 84908-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 84908-2-RR (same clone as 84908-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84908-2-RR (same clone as 84908-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 84908-2-RR (same clone as 84908-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84908-2-RR (same clone as 84908-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84908-2-RR, Clone: 241942D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 84908-2-RR (same clone as 84908-2-PBS)

  Dot blot analysis was used to confirm the specificity of 84908-2-RR Di/Tri-Methyl-Histone H3 (Lys4) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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Product Information

84908-2-PBS targets Di/Tri-Methyl-Histone H3 (Lys4) in WB, IHC, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H3a
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.