Di-Methyl-Histone H4 (Arg3) Recombinant monoclonal antibody

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• Di-Methyl-Histone H4 (Arg3) Recombinant monoclonal antibody (82859-1-RR)

• 

  WB analysis using 82859-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 82859-1-RR

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  Dot Blot experiment of peptide using 82859-1-RR

  Dot blot analysis was used to confirm the specificity of 82859-1-RR Di-Methyl-Histone H4 (Arg3) antibody. Peptides were spotted onto NC and probed with antibody at 5 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

• 

  Dot Blot experiment of peptide using 82859-1-RR

  Dot blot analysis was used to confirm the specificity of 82859-1-RR Di-Methyl-Histone H4 (Arg3) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

Di-Methyl-Histone H4 (Arg3) Uni-rAb^® Recombinant Antibody for WB, Dot Blot, ELISA

Cat No. 82859-1-RR

Clone No.1E21

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Dot Blot, ELISA

Synonyms

Di Methyl Histone H4, Di-Methyl Histone H4 (Arg3), Di-Methyl Histone H4 Arg3, Di-Methyl Histone-H4 Arg3, Di-Methyl-Histone H4

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Tested Applications

• Positive WB detected in: THP-1 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells
• Positive Dot Blot detected in: peptide

This antibody specifically recognize symmetric dimethyl-arginine (SDMA, Rme2s).

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

82859-1-RR targets Di-Methyl-Histone H4 (Arg3) in WB, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H4a
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC069654
• Gene Symbol: HIST1H4A
• Gene ID (NCBI): 8359
• RRID: AB_3670583
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62805
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Arginine dimethylation is an important post-translational modification of proteins. Based on the positions of the two methyl groups on the guanidino group of arginine, it can be divided into symmetric dimethylation (SDMA) and asymmetric dimethylation (ADMA). Symmetric dimethylation is crucial for embryonic development and cell differentiation and is often associated with transcriptional repression. This antibody specifically recognizes symmetric dimethyl-arginine (SDMA, Rme2s).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Br J Cancer; Targeting gut microbiota and arginase boosts MEK inhibitors' enhancement of antitumour immunity via MHC-I upregulation in colorectal cancer.; Authors - Jingdan Zhang