Di-Methyl-Histone H4 (Arg3) Recombinant antibody

Di-Methyl-Histone H4 (Arg3) Uni-rAb^TM Recombinant Antibody for WB, ELISA, Dot Blot

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA, Dot Blot

Conjugate

Unconjugated

CloneNo.

1E21

Cat no : 82859-1-RR

Synonyms

H4/a, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4, histone cluster 1, H4a, Histone H4

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Validation Data Gallery

• 

  WB analysis using 82859-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 82859-1-RR

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  Dot Blot experiment of peptide using 82859-1-RR

  Dot blot analysis was used to confirm the specificity of 82859-1-RR Di-Methyl-Histone H4 (Arg3) antibody. Peptides were spotted onto NC and probed with antibody at 5 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

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Tested Applications

• Positive WB detected in: THP-1 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells
• Positive Dot Blot detected in: peptide

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82859-1-RR targets Di-Methyl-Histone H4 (Arg3) in WB, ELISA, Dot Blot applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H4a
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC069654
• Gene Symbol: HIST1H4A
• Gene ID (NCBI): 8359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.