Desmin Recombinant antibody, PBS Only

Desmin Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D21

Cat no : 82748-1-PBS

Synonyms

DES, 1D21

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Validation Data Gallery

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  WB analysis using 82748-1-RR (same clone as 82748-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82748-1-RR (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82748-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 82748-1-RR (same clone as 82748-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82748-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 82748-1-RR (same clone as 82748-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82748-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 82748-1-RR (same clone as 82748-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82748-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human normal colon using 82748-1-RR (same clone as 82748-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82748-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 82748-1-RR (same clone as 82748-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82748-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82748-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82748-1-RR against Human Desmin were performed. The affinity constant is 1.32 nM.

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Product Information

82748-1-PBS targets Desmin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Desmin fusion protein Ag9742
• Full Name: desmin
• Calculated Molecular Weight: 470 aa, 54 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC032116
• Gene Symbol: Desmin
• Gene ID (NCBI): 1674
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.