DYSFIP1 Polyclonal antibody

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  WB analysis using 18208-1-AP

  Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18208-1-AP (DYSFIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 18208-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using DYSFIP1 antibody (18208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DYSFIP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 18208-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Toonin, Protein phosphatase 1 regulatory subunit 27, PPP1R27, Dysferlin-interacting protein 1

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Tested Applications

• Positive WB detected in: rat skeletal muscle tissue
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18208-1-AP targets DYSFIP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYSFIP1 fusion protein Ag12839
• Full Name: dysferlin interacting protein 1
• Calculated Molecular Weight: 154 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC093707
• Gene Symbol: DYSFIP1
• Gene ID (NCBI): 116729
• RRID: AB_3669295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86WC6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DYSFIP1 is also known as PPP1R27. DYSFIP1 inhibits phosphatase activity of protein phosphatase 1 (PP1) complexes. The molecular weight of DYSFIP1 is 17 kDa.