DYRK2 Polyclonal antibody

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  WB analysis using 21979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21979-1-AP (DYRK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 21979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using DYRK2 antibody (21979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DYRK2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 21979-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: HL-60 cells, K-562 cells, mouse testis tissue, rat testis tissue
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21979-1-AP targets DYRK2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYRK2 fusion protein Ag13480
• Full Name: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67~70 kDa
• GenBank Accession Number: BC006375
• Gene Symbol: DYRK2
• Gene ID (NCBI): 8445
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92630
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DYRK (Dual-specificity tyrosine phosphorylation-regulated kinase 2) is the only member of the Dual-specificity tYrosine phosphorylation-Regulated Kinase (DYRK) family that functions as a kinase activity-independent scaffold for an E3 ubiquitin ligase complex (PMID: 33376136). DYRK2 is involved in cell survival, development, differentiation, gene transcription, proteasomal regulation, and microtubule formation. DYRK2 is a key regulator of cell cycle and apoptosis in response to DNA damage via phosphorylation of p53. DYRK2 has two isoforms and is mostly localized in the cytoplasm; however, it accumulates in the nucleus under genotoxic stress conditions (PMID: 31505048, 37886981).