DYRK1A Polyclonal antibody

DYRK1A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28607-1-AP

Synonyms

DYRK, DYRK1, DYRK1A, hMNB, HP86, MNB, MNBH

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Validation Data Gallery

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  WB analysis using 28607-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28607-1-AP (DYRK1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 28607-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 28607-1-AP (DYRK1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 28607-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 28607-1-AP (DYRK1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-3 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28607-1-AP targets DYRK1A in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYRK1A fusion protein Ag29433
• Full Name: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 70kDa, 85 kDa
• GenBank Accession Number: NM_001396
• Gene Symbol: DYRK1A
• Gene ID (NCBI): 1859
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome.