DYNLT3 Polyclonal antibody

DYNLT3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11687-1-AP

Synonyms

TCTEX1L, TCTE1XL, TCTE1L, T-complex-associated testis-expressed 1-like, RP3

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Validation Data Gallery

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  WB analysis of U-937 using 11687-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11687-1-AP (DYNLT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11687-1-AP (DYNLT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 11687-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11687-1-AP (DYNLT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11687-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11687-1-AP (DYNLT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11687-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DYNLT3 antibody (11687-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: U-937 cells, HeLa cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11687-1-AP targets DYNLT3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DYNLT3 fusion protein Ag2299
• Full Name: dynein, light chain, Tctex-type 3
• Calculated Molecular Weight: 116 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC000968
• Gene Symbol: DYNLT3
• Gene ID (NCBI): 6990
• RRID: AB_2093777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dynein light chain Tctex-type 3 (DYNLT3) is a member of the cytoplasmic dynamic protein light chain Tctex family. DYNLT3 mainly plays a regulatory role in mitosis and meiosis, as well as chromosome binding. DYNLT3 is expressed in all cells and tissues. The calculated molecular weight of DYNLT3 is 13 kDa.