ChromoTek DYKDDDDK Fab-Trap™ Agarose Kit

The ChromoTek DYKDDDDK Fab-Trap™ Agarose Kit consists of an anti-DYKDDDDK-tag Fab fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap™ Agarose Kit is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc

Specificity

Flag®-tag (1x and 3x)

Applications

IP, CoIP, ChIP, RIP, Protein purification

Type

Fab-fragment

Conjugate

Agarose

Cat no : ffak

Synonyms

Fab-Trap Agarose, Fab-Trap, DYKDDDDK, flag



Product Information

The ChromoTek DYKDDDDK Fab-Trap™ Agarose Kit consists of an anti-DYKDDDDK-tag Fab fragment, which is covalently bound to agarose beads. DYKDDDDK Fab-Trap™ Agarose Kit is used to immunoprecipitate 1x and 3xDYKDDDDK-tagged fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc

DescriptionThe DYKDDDDK Fab-Trap Agarose Kit contains DYKDDDDK Fab-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of Flag®-tagged proteins and their interacting factors.

• Lowest background in class

• Less contaminating antibody chains

• Recombinantly expressed Fab-fragment derived from M2 clone

• Compatible with harsh buffer conditions

• Ready-to-use

ApplicationsIP, CoIP, ChIP, RIP, Protein purification
Specificity/TargetThe DYKDDDDK Fab-Trap™ Agarose specifically binds to 1x and 3xDYKDDDDK-tag sequence (1x and 3xFlag®-tag). It binds to same epitope as Sigma's Anti-FLAG® M2 Antibody and is compatible with N-, C-terminal or internal tagging.
Binding capacity12.5 - 15 µg of recombinant 3xDYKDDDDK-tagged protein (~30 kDa) per 25µL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution buffer3xDYKDDDDK-peptide
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility0.5 % Nonidet P40 Substitute, 5 % Tween™ 20, 2 M NaCl, 10 mM TCEP, 10 mM DTT, 10 mM ß-mercaptoethanol, 2 M Urea
Type Fab-fragment
ClassRecombinant
HostMouse
RRIDAB_2894836
Storage Buffer1x PBS, Preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

Component Description
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash bufferRemoval of unwanted proteins, peptides, etc.
DYKDDDDK Fab-Trap™ Agarose20 reactions (500 µL slurry)