DVL3 Polyclonal antibody

DVL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 13444-1-AP

Synonyms

Dishevelled 3, Segment polarity protein dishevelled homolog DVL-3, KIAA0208, DSH homolog 3, Dishevelled-3

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Validation Data Gallery

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  WB analysis using 13444-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13444-1-AP (DVL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13444-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13444-1-AP (DVL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 13444-1-AP

  IP result of anti-DVL3 (IP:13444-1-AP, 4ug; Detection:13444-1-AP 1:500) with Raji cells lysate 1440ug.

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  IHC staining of human colon cancer using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 13444-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13444-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DVL3 antibody (13444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13444-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13444-1-AP (DVL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human colon cancer tissue, human prostate cancer tissue, mouse colon tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

13444-1-AP targets DVL3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, xenopus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DVL3 fusion protein Ag4272
• Full Name: dishevelled, dsh homolog 3 (Drosophila)
• Calculated Molecular Weight: 716 aa, 78 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC032459
• Gene Symbol: DVL3
• Gene ID (NCBI): 1857
• RRID: AB_2093451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
Xenopus	WB,IP	J Cell Biol; Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled.; Authors - Moonsup Lee
mouse	IF	J Cell Biol; Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling.; Authors - Andre Landin Malt
human	WB	Oncogene; The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.; Authors - Kai Li
human	WB	Cancer Lett; SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/β-catenin signal network.; Authors - Li Xuefei X
human	WB	Oncotarget; Dalbinol, a rotenoid from Amorpha fruticosa L., exerts anti-proliferative activity by facilitating β-catenin degradation in hepatocellular carcinoma cells.; Authors - Xiaohui Zhu
human	WB,IHC,IF	Biol Proced Online; Circadian gene CSNK1D promoted the progression of hepatocellular carcinoma by activating Wnt/β-catenin pathway via stabilizing Dishevelled Segment Polarity Protein 3; Authors - Mengqi Zhu